Histology

Histology is the study of the microscopic structure of tissues in the human body. It involves the examination of tissues at the cellular level and the identification of their functions. There are four basic types of tissue in the human body: epithelial, connective, muscle, and nervous tissue.

Epithelial tissue is composed of closely packed cells that form a thin, continuous layer. It covers the surface of the body, lines body cavities, and forms glands. Epithelial tissue is classified by the number of cell layers and the shape of the cells. Simple epithelium is a single layer of cells, while stratified epithelium is composed of two or more layers of cells. Squamous cells are thin and flat, cuboidal cells are cube-shaped, and columnar cells are tall and cylindrical.

Connective tissue is a diverse group of tissues that provide support and protection to the body. It includes bone, cartilage, blood, adipose tissue, and fibrous connective tissue. Connective tissue is characterized by its extracellular matrix, which is composed of fibers and ground substance. The fibers can be collagen, elastic, or reticular, and the ground substance is a fluid or gel-like substance that surrounds the cells and fibers.

Muscle tissue is responsible for movement and force generation in the body. There are three types of muscle tissue: skeletal, cardiac, and smooth muscle. Skeletal muscle is attached to bones and is under voluntary control. It is composed of long, cylindrical cells called muscle fibers. Cardiac muscle is found in the heart and is responsible for pumping blood. It is composed of short, branched cells called cardiomyocytes. Smooth muscle is found in the walls of organs and is responsible for involuntary movement. It is composed of spindle-shaped cells.

Nervous tissue is responsible for transmitting and processing information in the body. It includes the brain, spinal cord, and nerves. Nervous tissue is composed of two types of cells: neurons and glial cells. Neurons are specialized cells that transmit electrical signals, and glial cells provide support and nourishment to the neurons.

Epithelial Tissue 

Epithelial tissues are one of the four primary tissue types in the human body, alongside connective, muscular, and nervous tissues. They are made up of closely packed cells that form a continuous sheet or layer, covering the internal and external surfaces of the body. The following is a detailed explanation of the different types of epithelial tissues found in the human body.

Figure 19: Epithelial Tissue Types

Figure 20: Simple squamous epithelium from lung

Simple squamous epithelium tissue is composed of a single layer of flat, scale-like cells. It is found lining the alveoli of the lungs, the walls of blood vessels, and the serous membranes that line the body cavities. Simple squamous epithelium is well suited for diffusion and filtration because of its thin structure and the ease with which substances can pass through it. 

Figure 21: Keratinized stratified squamous epithelium from skin 

Stratified squamous epithelium consists of several layers of flattened cells. It is found on the surface of the skin, the lining of the mouth, esophagus, and vagina. Stratified squamous epithelium provides protection against abrasion, dehydration, and microbial invasion. 

Keratinized stratified squamous epithelium  contains dead cells full of the keratin protein, causing thickened skin and water-proofing. 

Non-Keratinized stratified squamous epithelium contains alive cells in many layers meant to protect moist tissues.

Figure 22: Non-Keratinized stratified squamous epithelium from cervix

Figure 23: Simple cuboidal epithelium from thyroid

Simple cuboidal epithelium is made up of a single layer of cube-shaped cells. It is found in the lining of the kidney tubules, the thyroid gland, and the ducts of many other glands. Simple cuboidal epithelium is involved in secretion and absorption.

Figure 24: Stratified cuboidal epithelium from parotid salivary gland

Stratified cuboidal epithelium is composed of several layers of cube-shaped cells. It is found in the ducts of sweat glands, mammary glands, and salivary glands. Stratified cuboidal epithelium provides protection and helps to maintain the shape of the ducts.

Figure 25: Simple columnar epithelium from intestines

Simple columnar epithelium is made up of a single layer of tall, thin cells. It is found lining the gastrointestinal tract, the gallbladder, and some ducts of glands.  Simple columnar epithelium is involved in absorption, secretion, and protection. 

Villi are rippled or wavy tissues made of cells, to increase surface area of absorptive tissues.

Microvilli are modifications to the surface of the cells that increase the surface area of the cell membrane.

Cilia are long membrane bound protrusions from a cell that will add in movement of fluids and substances around a cell or tissue.

Figure 26: Pseudostratified columnar epithelium from esophagus

Pseudostratified columnar epithelium appears to be stratified but is actually composed of a single layer of cells with nuclei at different levels. It is found lining the respiratory tract, including the trachea and bronchi. Pseudostratified columnar epithelium helps to protect the respiratory system and is involved in the movement of mucus and other substances. 

Figure 27: Stratified columnar epithelium from urethra

Stratified columnar epithelium consists of multiple layers of cells with a columnar shape. It is primarily found in areas where the tissue requires protection and secretion, such as the lining of the male urethra, large ducts of some glands, and parts of the pharynx. The cells in the superficial layer are typically elongated and column-shaped, while the cells in the deeper layers are cuboidal or polyhedral in shape. This tissue is characterized by its ability to provide a barrier against mechanical and chemical stresses, while also allowing for the secretion and absorption of substances. Stratified columnar epithelium can also undergo changes in response to different stimuli, such as inflammation or hormonal influences. 

Figure 28: Transitional epithelium from urinary bladder

Transitional epithelium is found in organs that stretch and change shape, such as the bladder and ureters. It is composed of several layers of cells that can change shape and size in response to tension. Transitional epithelium allows these organs to expand and contract without tearing. 

Epithelial tissues are essential for the proper functioning of many organs and structures in the body. They provide protection, absorption, and secretion, and help to regulate the exchange of substances between the body and its environment.

Connective Tissue

Connective tissues are the most abundant tissues in the body, and they provide structural support and maintain the integrity of the body's organs and tissues. They are classified into four categories: proper dense connective tissue, proper loose connective tissue, cartilage, and blood.

Proper Dense Connective Tissue

This type of connective tissue consists of densely packed collagen fibers that provide strength and resistance to stress. It is divided into two subtypes: regular and irregular.

Figure 29: Regular Dense Connective Tissue from tendon

Regular Dense Connective Tissue, also known as white fibrous tissue, has collagen fibers arranged in a parallel manner, giving it high tensile strength in one direction. It is found in tendons, which connect muscles to bones, and in ligaments, which connect bones to other bones. 

Figure 30: Irregular Dense Connective Tissue from skin

Irregular Dense Connective Tissue has collagen fibers arranged in a random manner, giving it strength in multiple directions. It is found in the dermis of the skin, the outer covering of organs, and the capsule surrounding joints. 

Figure 31: Elastic Connective Tissue from skin

Elastic Connective Tissue, also known yellow elastic tissue, as is rich in elastic fibers, giving it the ability to stretch and recoil. It's found in arteries, the lungs, and skin.  

Proper Loose Connective Tissue

This type of connective tissue consists of a loose arrangement of collagen, elastic, and reticular fibers, and it serves as a support structure for organs and tissues. 

Figure 32: Areolar Tissue

Areolar Tissue is the most widely distributed type of connective tissue, and it forms a soft packing material around organs, blood vessels, and nerves. 

Figure 33: Adipose Tissue

Adipose Tissue is composed of adipocytes, which store fat. It is found in the subcutaneous layer of the skin, around organs, and in bone marrow. 

Figure 34: Reticular Tissue 

Reticular Tissue contains reticular fibers, which form a mesh-like network. It is found in the spleen, lymph nodes, and bone marrow. 

Cartilage

Cartilage is a firm, flexible connective tissue that provides support and shock absorption to joints and other structures. It is divided into three types: hyaline, elastic, and fibrocartilage. 

Figure 35: Hyaline Cartilage

Hyaline Cartilage has a smooth, glassy appearance and is found at the ends of bones in joints, the nose, and the trachea. 

Figure 36: Elastic Cartilage

Elastic Cartilage has a yellowish appearance and is found in the external ear and the epiglottis. 

Figure 37: Fibrocartilage

Fibrocartilage is a combination of dense connective tissue and cartilage, and it is found in the intervertebral discs, the pubic symphysis, and the menisci of the knee. 

Bone

Bone connective tissue is a specialized type of connective tissue that is composed of cells, fibers, and extracellular matrix (ECM) that give it its unique characteristics. Bones provide support, protection, and movement to the body, and are composed of two types of bone tissue: compact bone and spongy bone. 

Figure 38: Compact Bone

Compact Bone, also known as cortical or ground bone, is the dense and strong outer layer of bone that makes up the shafts of long bones and the outer surfaces of other bones. It is organized into functional units called osteons or Haversian systems, which are cylindrical structures consisting of concentric layers of mineralized matrix called lamellae. Each osteon contains a central canal, the Haversian canal, which houses blood vessels, lymphatic vessels, and nerves that supply the bone cells with nutrients and oxygen. The lamellae are arranged in a parallel fashion around the Haversian canal, and the cells that make up the bone tissue, called osteocytes, are located in spaces called lacunae between the lamellae. Osteocytes communicate with each other and with the central canal through tiny channels called canaliculi. Compact bone is also characterized by its numerous small spaces called perforating canals, which connect the Haversian canals to the outer surface of the bone and allow for the passage of blood vessels and nerves. 

Figure 39: Spongy Bone

Spongy Bone, also known as cancellous or trabecular bone, is the less dense and more porous inner layer of bone that is found at the ends of long bones and in the center of other bones. It consists of a network of bony struts called trabeculae, which provide support and help to reduce the weight of the bone. The spaces between the trabeculae are filled with bone marrow, which is responsible for producing blood cells. Spongy bone also contains lamellae, osteocytes, and canaliculi, but they are arranged differently than in compact bone. Instead of being organized around Haversian canals, they are arranged along lines of stress within the bone, and the canaliculi interconnect the osteocytes in the trabeculae with those in the bone marrow. 

Bone connective tissue is a specialized type of tissue that provides support, protection, and movement to the body. It is composed of two types of bone tissue, compact bone and spongy bone, each with its own unique structure and function. Both types of bone tissue are composed of cells, fibers, and ECM that work together to maintain the integrity and strength of the bone.  Both compact and spongy bone are composed of the same basic components: osteocytes, osteoblasts, osteoclasts, and ECM. Osteocytes are mature bone cells that are responsible for maintaining the bone tissue by communicating with other osteocytes and with bone-forming cells called osteoblasts. Osteoblasts are responsible for synthesizing and depositing new bone matrix, while osteoclasts are responsible for breaking down and resorbing old bone tissue. The ECM of bone is composed of collagen fibers, which provide tensile strength to the bone, and hydroxyapatite crystals, which provide hardness and rigidity. 

Figure 40: Blood Connective Tissue

Blood

Blood is a fluid connective tissue that circulates throughout the body, carrying nutrients, oxygen, and waste products. It is composed of cells, including red blood cells, white blood cells, and platelets, and a liquid matrix called plasma. 

Muscle

There are three main types of muscle tissue found in the human body: skeletal, smooth, and cardiac. Each type of muscle tissue possesses unique structural and functional characteristics. 

Figure 41: Skeletal Muscle Tissue

Skeletal Muscle Tissue is a type of striated muscle tissue that is attached to bones by tendons. It is responsible for voluntary movement and is under conscious control. The cells of skeletal muscle tissue are long, cylindrical, and multinucleated, with striations or stripes that give the muscle its characteristic banded appearance. These cells are known as muscle fibers and are made up of myofibrils, which contain the contractile proteins actin and myosin. Skeletal muscle fibers are innervated by somatic motor neurons, which release acetylcholine at the neuromuscular junction, causing the muscle to contract. Skeletal muscle fibers also contain specialized structures called T-tubules and sarcoplasmic reticulum, which play important roles in the regulation of muscle contraction. 

Figure 42: Smooth Muscle Tissue

Smooth Muscle Tissue is found in the walls of hollow organs such as the stomach, intestines, uterus, and blood vessels. It is responsible for involuntary movements such as peristalsis and vasoconstriction. Unlike skeletal muscle tissue, smooth muscle tissue is not striated and is under autonomic control.

Smooth muscle cells are spindle-shaped and contain a single nucleus. The cells are organized into sheets or layers and are connected by gap junctions, allowing for coordinated contraction. Smooth muscle cells contain contractile proteins called actin and myosin, but they are arranged differently than in skeletal muscle tissue. Smooth muscle cells are innervated by autonomic neurons that release either acetylcholine or norepinephrine, depending on the type of receptor present on the muscle cell. This leads to either contraction or relaxation of the muscle, depending on the type of neurotransmitter released. 

Figure 43: Cardiac Muscle Tissue

Cardiac Muscle Tissue is found only in the heart and is responsible for the involuntary pumping of blood. Like skeletal muscle tissue, cardiac muscle tissue is striated, but it is under involuntary control. Cardiac muscle cells, also called cardiomyocytes, are branched and contain a single nucleus. They are interconnected by specialized structures called intercalated discs, which allow for synchronized contraction of the heart. Cardiomyocytes contain myofibrils with actin and myosin filaments, similar to skeletal muscle tissue, but they also contain structures called intercalated discs that facilitate communication and electrical conduction between cells. Cardiac muscle tissue is innervated by the autonomic nervous system, but the regulation of heart rate and force of contraction is primarily controlled by the sinoatrial node, a specialized group of cells in the heart that acts as the pacemaker. The sinoatrial node generates electrical impulses that spread throughout the heart, causing coordinated contraction of the cardiac muscle tissue. 

Figure 44: Nervous tissue

Nervous

Nervous tissue is a specialized type of tissue that is found in the nervous system. It is composed of two primary types of cells: neurons and neuroglia (also known as glial cells).

Neurons are the functional unit of the nervous system and are responsible for transmitting nerve impulses. They are composed of three main parts: the cell body, dendrites, and axons. The cell body contains the nucleus and other organelles necessary for the neuron's metabolic functions. Dendrites are the receiving end of the neuron and are responsible for receiving signals from other neurons. Axons are the transmitting end of the neuron and are responsible for transmitting signals to other neurons or to muscles and glands.

Neuroglia, on the other hand, provide structural and functional support for neurons. They are divided into four main types: astrocytes, oligodendrocytes, microglia, and ependymal cells. Astrocytes are star-shaped cells that help regulate the chemical environment around neurons. Oligodendrocytes form myelin sheaths around axons, which helps to increase the speed of nerve impulse transmission. Microglia act as the immune cells of the nervous system, defending it against infection and injury. Ependymal cells line the ventricles of the brain and produce cerebrospinal fluid.

Nervous tissue can be further classified into two main types: gray matter and white matter. Gray matter is composed of neuron cell bodies, dendrites, and unmyelinated axons. It is responsible for processing and integrating information in the nervous system. White matter, on the other hand, is composed of myelinated axons and is responsible for transmitting nerve impulses between different regions of the nervous system. 

Overview

Histology is the study of the microscopic structure of tissues in the human body, examining tissues at the cellular level and identifying their functions. The four basic types of tissue are epithelial, connective, muscle, and nervous tissue, each with unique structures and functions contributing to the overall function of the body.

Epithelial tissue is composed of closely packed cells forming a thin, continuous layer that covers the body's surface, lines body cavities, and forms glands. It is classified by the number of cell layers (simple or stratified) and cell shapes (squamous, cuboidal, or columnar). Epithelial tissue plays a vital role in protection, absorption, and secretion, regulating the exchange of substances between the body and its environment.

Connective tissue is a diverse group of tissues providing support and protection to the body. It includes bone, cartilage, blood, adipose tissue, and fibrous connective tissue. Connective tissue is characterized by its extracellular matrix, composed of fibers (collagen, elastic, or reticular) and ground substance (fluid or gel-like substance surrounding cells and fibers). Connective tissues maintain the integrity of the body's organs and tissues, providing structural support.

Muscle tissue is responsible for movement and force generation in the body. There are three types of muscle tissue: skeletal, cardiac, and smooth muscle. Skeletal muscle is attached to bones and is under voluntary control, composed of long, cylindrical cells called muscle fibers. Cardiac muscle is found in the heart, responsible for pumping blood, and is composed of short, branched cells called cardiomyocytes. Smooth muscle is found in the walls of organs and is responsible for involuntary movement, composed of spindle-shaped cells.

Nervous tissue is responsible for transmitting and processing information in the body, including the brain, spinal cord, and nerves. Nervous tissue is composed of two types of cells: neurons and glial cells. Neurons are specialized cells that transmit electrical signals, while glial cells provide support and nourishment to neurons.

Understanding histology is essential for comprehending the structure and function of the human body. Each type of tissue has a unique structure and function, allowing it to perform its specialized role in the body. Epithelial tissue provides protection, absorption, and secretion; connective tissue offers support and protection; muscle tissue enables movement and force generation; and nervous tissue facilitates the transmission and processing of information throughout the body.